Based on the LC-MS / MS platform, Creative Proteomics use analytical standard detection methods to perform accurate qualitative and quantitative analysis on the pre-screened and validated or identified potential biomarkers (chlorophyll b).
Molecular structure of chlorophyll b
Chlorophyll b is a chemical substance whose molecular formula is C55H70MgN4O6, and CAS number is 519-62-0.
Chlorophyll b is a kind of chlorophyll, which absorbs and transmits light energy as one of the antenna pigments of photosynthesis.
Chlorophyll b exists in higher plants, green algae, Euglena and tube algae.
Chlorophyll b has one more carbonyl group than chlorophyll a, so it is more soluble in polar solvents.
The color of chlorophyll b is yellow-green, mainly absorbing blue-violet light.
The maximum absorption wavelength of chlorophyll b is 460-645nm, the emission wavelength is 469nm, and the excitation wavelength is 670nm.
Targeted metabolomics based on mass spectrometry can more sensitively and more accurately quantify target metabolites. This method is mainly used to identify and quantitatively analyze specific metabolites, and has been widely used to analyze and compare multiple target metabolites in different physiological states. It is a key analysis method for researches on green plants to improve nutrition and so on. With decades of experience in mass spectrometry services, Creative Proteomics undertakes the qualitative and quantitative analysis of a variety of plant metabolites, provides accurate and detailed data and analysis reports. From sample extraction and detection to bioinformatics, Creative Proteomics can meet your special needs, and has a good record.
Algae samples need to prepare 30-40mg freeze-dried powder or 300mg fresh samples.
Plant tissues need to be mixed from more than 3 plants as a sample.
Plant samples need to be freeze-dried, please try to provide more than 3g (fresh weight).
The samples should be stored at -80°, transported on dry ice, and cannot be frozen and thawed repeatedly.
Biological replicates required
If it is a plant or microorganism sample, no less than 6 biological replicates.
Mass spectrometry detection of standard and standard curve preparation
Mass spectrometry detection of samples
Raw data preprocessing
Absolute quantification of target metabolites
Analysis of differences between groups
Extended analysis, such as Metabolite screening, KEGG pathway analysis and hierarchical clustering
About 1-3 weeks
Creative Proteomics has leading technology, first-class R&D and production equipment, and has established a complete plant targeted metabolome service system, making the upstream and downstream experiments seamlessly connected. You can also perform personalized customization services at the same time in the data analysis stage. It also has high-quality after-sales service to help you analyze the project's final report and help you answer your doubts. If you want to know more, please contact us immediately.
For Research Use Only. Not for use in diagnostic procedures.