The three carotenoids, antheraxanthin and zeaxanthin and violaxanthin, can undergo reversible interactions in the phytoalexin cycle. Antheraxanthin is the product of the first epoxidation reaction catalyzed by zeaxanthin epoxidase, and its metabolism plays an important role in phytohormone biosynthesis. The intermediate product isopentenyl pyrophosphate is converted to gibberellins, cytokinins and abscisic acid under different conditions, and carotenoids are also formed.
In the human body, antheraxanthin, like many carotenoids, protects against cell and organ damage caused by free radicals in the body and has anticancer and cardiovascular disease prevention effects.
Creative Proteomics has established HPLC and LC-MS/MS analytical methods for the accurate and rapid identification and quantification of antheraxanthin to help you accelerate your project.
Structure of antheraxanthin
Detection Platform
- HPLC:
High performance liquid chromatography allows the determination of various components in extract samples, including various carotenoids, such as lutein and zeaxanthin. This method is highly sensitive, e.g. fluorescence detector sensitivity up to 10-11 g, detection limit up to 109, injection volume in the order of μL, and quantitative detection is not affected by impurities in the extracts.
- LC-MS/MS: Q Exactive LC-MS/MS
The Q Exactive mass spectrometer combines the parent ion selectivity of a high-performance quadrupole with the high-resolution accurate mass number (HR/AM) Orbitrap detection technology to provide excellent performance and outstanding versatility for qualitative and quantitative compound detection with high sensitivity and throughput compared to HPLC.
Technical Workflow of Targeted Metabolomics of Antheraxanthin
Sample Preparation Suggestions
| Sample type | Suggested sample quantity (per case) | Number of repetitions (recommended) |
|---|
| Plant tissue | ≥ 1g | ≥ 6 |
| Animal and clinical tissues | ≥ 200 mg | ≥ 10 |
| Serum, plasma | ≥ 500 μl | ≥ 6 |
| Urine | ≥ 200 μl | ≥ 6 |
| Stool, intestinal contents | ≥ 200 mg | ≥ 6 |
| Cell | ≥ 107 | ≥ 6 |
| Microorganism | ≥ 107 or ≥ 100 mg | ≥ 6 |
| Culture broth, fermentation broth | ≥ 1 ml | ≥ 6 |
※ There is no restriction on sample varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.
Feedback to Customers
- A detailed technical report will be provided at the end of the entire project, including experimental procedures and instrument parameters.
- The report also includes quantitative results of targeted metabolites.
- Part of the mass spectrum picture
- Raw data
Project Cycle
- A standard experimental procedure takes about 1 ~ 4 weeks.
Creative Proteomics has an integrated separation, detection, identification and quantification system, with excellent robustness and reproducibility, high sensitivity and ultra-sensitivity, and can provide fast and reliable targeted metabolomics services. Our one-stop service model can help you achieve a seamless connection between upstream and downstream experiments. Coupled with personalized analysis services, your analysis can reach the final research purpose. If you have any questions or specific requirements, please feel free to contact us.
For Research Use Only. Not for use in diagnostic procedures.
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