Creative Proteomics' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results. CPR is based on LC-MS/GC-MS technology, using target compound standards for method optimization, method validation, biological sample collection, metabolite extraction, mass spectrometry detection, data analysis, etc.
Lycopene is a carotenoid found in plant foods, and it is also a red pigment. It is widely found in tomatoes, carrots, watermelon, papaya, guava, grapefruit and other fruits.
Lycopene does not have the β-carotene ring structure of β-pondroitone, so it cannot be converted into vitamin A in the body. Lycopene, which does not have the physiological activity of vitamin A, has a strong antioxidant function, and is also often used as a raw material for antioxidant health foods.
At present, lycopene has not only been widely used as a natural pigment, but also has been increasingly used in functional foods, medicines and cosmetics.
Lycopene is an unsaturated olefin compound with a molecular formula of C40H56. There are 11 conjugated double bonds and 2 non-conjugated double bonds in the molecular structure, forming a linear hydrocarbon compound. There are many cis-trans isomers. The CAS number is 502-65-8.
MS-based technical means can realize the qualitative and quantitative analysis of lycopene.
Structure of lycopene
Waters ACQUITY UPLC, MS-SCIEX QTRAP 4500/5500/6500
Agilent 7890B-5977A, Thermo TRACE 1300-TSQ 9000
Technical Workflow of Targeted Metabolomics of Lycopene
- Standard curve creation
- Raw data preprocessing
- Absolute quantitation of lycopene
Sample Preparation Suggestions
|Sample type||Suggested sample quantity (per case)||Number of repetitions (recommended)|
|Plant tissue||≥ 1g||≥ 6|
|Animals and clinical organizations||≥ 200 mg||≥ 10|
|Algae samples||≥ 30mg freeze-dried powder / 300mg fresh samples||≥ 6|
|Serum, plasma||≥ 200 mg||≥ 6|
|Urine||≥ 200 μl||≥ 6|
|Stool, intestinal contents||≥ 500 μl||≥ 6|
|Cell||≥ 107||≥ 6|
※ There is no restriction on sample varieties. For varieties with special requirements or rare varieties, please contact us for more information.
After collecting the samples, they were quickly frozen in liquid nitrogen, and then transferred to -80°C for storage. Transported on dry ice.
Feedback to Customers
- A detailed technical report will be provided at the end of the entire project, including experimental procedures and instrument parameters.
- The report also includes quantitative results of targeted metabolites, such as name, abbreviation, molecular formula, molecular weight and CAS number of the analyte.
- Part of the mass spectrum picture
- Raw data
- A standard experimental procedure takes about 1 ~ 4 weeks.
Creative Proteomics has an integrated separation, detection, identification and quantification system, with excellent robustness and reproducibility, high sensitivity and ultra-sensitivity, and can provide fast and reliable targeted metabolomics services. Our one-stop service model can help you achieve a seamless connection between upstream and downstream experiments. Coupled with personalized analysis services, your analysis can reach the final research purpose. If you have any questions or specific requirements, please feel free to contact us.
For Research Use Only. Not for use in diagnostic procedures.