Creative Proteomics' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results. CPR is based on LC-MS/GC-MS technology, using target compound standards for method optimization, method validation, biological sample collection, metabolite extraction, mass spectrometry detection, data analysis, etc.
Thapsigargin is extracted from thapsiagarganica.
Thalassene is classified as guaiacolactone.
It acts as an effective, cell-permeable, IP3-independent intracellular calcium release agent, and can prevent the transient increase of intracellular Ca2+ induced by angiostatin and endostatin.
It induces apoptosis by destroying the level of free Ca2+ in the cell.
The molecular formula of thapsigargin is C34H50O12, and the CAS number is 67526-95-8.
MS-based technical means can realize the qualitative and quantitative analysis of thapsigargin.
Structure of thapsigargin
Waters ACQUITY UPLC, MS-SCIEX QTRAP 4500/5500/6500
Agilent 7890B-5977A, Thermo TRACE 1300-TSQ 9000
Technical Workflow of Targeted Metabolomics of Thapsigargin
- Standard curve creation
- Raw data preprocessing
- Absolute quantitation of thapsigargin
Sample Preparation Suggestions
|Sample type||Suggested sample quantity (per case)||Number of repetitions (recommended)|
|Algae samples||≥ 30mg freeze-dried powder / 300mg fresh samples||≥ 6|
|Plant tissue||≥ 1g||≥ 6|
|Animals and clinical organizations||≥ 200 mg||≥ 10|
|Serum, plasma||≥ 200 mg||≥ 6|
|Urine||≥ 200 μl||≥ 6|
|Stool, intestinal contents||≥ 500 μl||≥ 6|
|Cell||≥ 107||≥ 6|
※ There is no restriction on sample varieties. For varieties with special requirements or rare varieties, please contact us for more information.
After collecting the samples, they were quickly frozen in liquid nitrogen, and then transferred to -80°C for storage. Transported on dry ice.
Feedback to Customers
- A detailed technical report will be provided at the end of the entire project, including experimental procedures and instrument parameters.
- The report also includes quantitative results of targeted metabolites, such as name, abbreviation, molecular formula, molecular weight and CAS number of the analyte.
- Part of the mass spectrum picture
- Raw data
- A standard experimental procedure takes about 1 ~ 4 weeks.
Creative Proteomics has an integrated separation, detection, identification and quantification system, with excellent robustness and reproducibility, high sensitivity and ultra-sensitivity, and can provide fast and reliable targeted metabolomics services. Our one-stop service model can help you achieve a seamless connection between upstream and downstream experiments. Coupled with personalized analysis services, your analysis can reach the final research purpose. If you have any questions or specific requirements, please feel free to contact us.
For Research Use Only. Not for use in diagnostic procedures.