Resveratrol Analysis Service

Resveratrol is a non-flavonoid polyphenolic organic compound, which is an antitoxin produced by many plants when they are stimulated. Resveratrol has antioxidant, anti-inflammatory, anticancer and cardiovascular protective effects. It can be synthesized in grape leaves and skins and is a biologically active ingredient in wine and grape juice. Resveratrol is a colorless needle crystal, and solubles in ether, chloroform, methanol, ethanol and acetone and other organic solvents. It can fluoresce under 366mm ultraviolet light, and can react with ferric trichloride -potassium ferricyanide. The maximum absorption wavelength of trans-resveratrol is 308nm, and that of cis-resveratrol is 288mm. When the buffer solution pH is low (pH1 to 7), trans-resveratrol remaines stable for several months in light protection, while cis-resveratrol remaines stable only in light protection and near neutral buffer solution. Because resveratrol has a wide range of uses, it is very important to establish the method for the analysis of resveratrol in plant metabolites.

High performance liquid chromatography (HPLC) is a simple, rapid, accurate and highly reproducible method to solve the problems associated with resveratrol analysis. Improved extraction and purification steps can also improve the sensitivity of the analytical method. creative Proteomics offers HPLC and LC-MS platforms to enable resveratrol qualitative and quantitative analysis services.

Applications of Resveratrol Analysis

• Qualitative analysis of the main types of resveratrol
• Quantitative analysis of resveratrol content
• Ensure safety and quality
• Provide basis for improvement

Advantages of Our Resveratrol Analysis Service

Project progress transparency, real-time tracking of progress. Customers can follow up all the trends of the project at any time, directly feedback to the project manager, solve the problem efficiently. In addition, online evaluation and improvement suggestions can be made for the service of various dimensions of the project.

Rich project experience. We have professional and experienced project management personnel and technical personnel, who can make scientific and efficient analysis plan according to customer needs.

Industry leading, multi – level and multi - selective analysis technology platform. We are equipped with industry-leading analytical experimental equipment, forming a strict standard operating process from sample extraction to computer analysis, which provides a stable and efficient hardware resource guarantee for the storage, processing and follow-up analysis of massive information and data.

Service Workflow

Our newly developed sample preprocessing method provides safety, environmental protection, rapidness, effectiveness, and the average recovery rate is more than 90%. Sample preprocessing at Creative Proteomics complies with the in-house standard operation procedures (SOP), ensuring maximized homogeneity between samples.

 Figure 1. Resveratrol analysis service workflow.

Mode phase: acetonitrile - water

Detector: UV detector

Linear range: r>0.999

Time: dozens of minutes, slightly different according to different ingredients

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantitation of resveratrol
• Differential metabolite screening
• Optimal analyses

Sample Requirements

1. Fresh tissues >1 g. If possible, samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.

2. Product samples.

At least 3 biological replicates.

Delivery

• Experimental procedure
• Parameters of HPLC
• Purity analysis report
• HPLC raw data files and HPLC data quality checks
• Metabolites quantification data
• Custom analysis report

HPLC based profiling of resveratrol enables quantitative analyses in a faster, more convenient, and more sensitive manner. With decades of experience in HPLC services, Creative Proteomics has a proven track record supporting diverse resveratrol detection and quantitation. We can meet your specific project requirements, from sample to bioinformatics.