Triglyceride (TAG) is an ester derived from glycerol and three fatty acid molecules. It is not only the main component of cell membranes, but also an important respiratory substrate. Bacteria have the ability to synthesize odd-numbered and branched-chain fatty acids. Most naturally occurring fats contain different triglycerides. In the intestinal tract, triglycerides are divided into monoacylglycerols and free fatty acids during lipolysis. The liver is the main organ for the synthesis and storage of triglycerides. When the human body needs fatty acids as an energy source, glucagon triggers the breakdown of triglycerides through sensitive lipase, releasing free fatty acids and providing energy for the body. In addition, elevated levels of human TAGs have been shown to be related to a variety of diseases, including atherosclerosis, heart disease, stroke, and metabolic syndrome.
In the food industry, fat analysis is closely related to the judgment of food fraud, the implementation of quality supervision, and the detection of food safety. Triglycerides are important substances in fat analysis. Based on HPLC technology, the composition of triglycerides and isomers in food can be analyzed. Enzymatic analysis and chiral-phase chromatography can locate fatty acids on the TAG molecule.
According to different analysis needs, Creative Proteomics provides customize exclusive triglyceride services solutions for you according to your samples.
HPLC is used to measure the content of various components in a sample. The liquid medium flows through the pipeline of the HPLC. After adding a sample to the instrument, the components of the sample are separated in the chromatographic column, and then the detector measures the content of each component.
LC methods, including capillary integral chromatography and ultra-high performance liquid chromatography, combined with mass spectrometry, can be ionized by electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) or atmospheric pressure photoionization (APPI).
The workflow of triglyceride analysis
Mode: SRM / MRM
- Standard curve creation
- Raw data preprocessing
- Absolute quantification of triglyceride
- Serum / plasma ≥ 100 ul/sample
- Tissues: ≥ 50 mg/sample
- Fats, oils, butter, flours, nuts, animal foods, mixes: ≥ 100g
Quick-freeze in liquid nitrogen and store at -80°C, and send it on dry ice.
Please contact us for other sample types and dosages.
- Experimental procedure
- Parameters of liquid chromatography / gas chromatography and MS
- MS raw data files and MS data quality checks
- Triglyceride quantification data
- Custom analysis report
Turnaround Time: 1-3 weeks
Creative Proteomics offers several approaches to provide triglyceride analysis service and deliver precise and detailed data and analysis report. If you want to detect other metabolites but have not found them, you can tell us through the inquiry form, and our technicians will communicate with you.
For Research Use Only. Not for use in diagnostic procedures.