Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Cinnamic Acid Analysis Service

Cinnamic Acid Analysis Service

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Creative Proteomics provides a method based on liquid chromatography mass spectrometer (LC-MS) for the analysis of plant tannins in plants, and provides sufficient robustness, sensitivity and high resolution for the identification and quantification of plant tannins. This high-throughput method consumes less time and can help researchers explore the role of plant tannins in plant development and their applications in industries such as agriculture and food safety.

Overview

Cinnamic acid, also known as β-phenylacrylic acid, is an organic acid isolated from cinnamon bark or benzoin. Cinnamic acid is mainly used for the preparation of esters, and can also be used as a plant growth promoter and long-acting fungicide. In plants, phenylalanine is deaminated and degraded to produce phenylacrylic acid. Mainly used in flavors and fragrances, food additives, pharmaceutical industry, daily chemicals, chemical industry, beauty, pesticides, organic synthesis,etc.

The chemical structure of natural and synthetic plant cinnamic acid and resistant plant cinnamic acid Figure 1. The chemical structure of natural and synthetic plant cinnamic acid and resistant plant cinnamic acid (Park 2020).

Detection Methods of Cinnamic acid:

  • Thin layer chromatography (TCL)
  • High Performance Liquid Chromatography (HPLC)
  • Liquid Mass Spectrometry (LC-MS)
  • Gas chromatography mass spectrometry (GC-MS)
  • Lon chromatography (LC)

Applications of Cinnamic Acid Analysis:

  • Ensure food safety assessment.
  • Crop improvement.
  • Reveal the mechanism of plant growth and metabolism.
  • Improve the understanding of plant cinnamic acid metabolism.

Advantages of Our Cinnamic Acid Analysis Service:

Flexible statistical analysis and bioinformatics analysis.

Fast turnaround times and affordable prices.

Rich experience in plant sample handling and cinnamic acid analysis.

Accurate detection and quantitation of cinnamic acid with HP-LC platforms.

Fast detection speed, simple detection method, practicality and high accuracy.

Service Workflow

Since different tissues have different matrix properties, Creative Proteomics has developed a variety of novel sample preparation techniques. Our service can analyze more than 100 kinds of plant cinnamic acid, including 1,3,5-tricaffeoylquinic acid, 1-caffeoylquinic acid, cinnamtannin A1, cinnamtanninA2, etc.

Cinnamic acid analysis service workflow       Figure 2. Cinnamic acid analysis service workflow.

Mode: MRM

Precision: pmol

Linear: R2 > 0.99

Analysis content:

  • Standard curve creation
  • Raw data preprocessing
  • Absolute quantitative and qualitative analysis of plant cinnamic acid
  • Differential metabolite screening
Detectable TanninsCASQuantification Methods
Cinnamic Acid621-82-9External reference method / isotope-labeled internal standard method

Sample Requirements

Ensure that the sample size is greater than 0.2 g or 0.2 mL.

Delivery

  • Experimental procedures
  • The parameters of liquid chromatograph and mass spectrometer
  • Extract mitochondria and purified protein samples
  • Purity analysis report
  • MS raw data files and MS data quality inspection
  • Metabolite quantitative data
  • Customized analysis report

The spectrum analysis of plant hormones based on mass spectrometry can quantitatively analyze plant cinnamic acid in a faster, more convenient and more sensitive way. With decades of experience in mass spectrometry services, Creative Proteomics has a good record in supporting the detection and quantitative analysis of a variety of plant hormones. We can meet your specific project requirements from samples to bioinformatics.

References

  1. Lee, YS, Son, E, et al. Synergistic uric acid-lowering effects of the combination of chrysanthemum indicum linne flower and cinnamomum cassia (l.) j. persl bark extracts. Evid Based Compl Alt. 2017.
  2. Park, Y. H. , Kim, D. H. , et al. A 12-week, multicenter, randomized, double-blind, placebo-controlled clinical trial for evaluation of the efficacy and safety of dkb114 on reduction of uric acid in serum. Nutrients, (2020), 12(12), 3794.
For Research Use Only. Not for use in diagnostic procedures.

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