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Cinnamic Acid Analysis Service

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What is Cinnamic Acid?

Cinnamic acid is a naturally occurring organic compound, a phenolic acid formed by the deamination of phenylalanine. It is an aromatic compound found in various plants, including cinnamon, balsam, and sage. It is used as a flavoring agent in foods such as candy, baked goods, and chewing gum. In addition to its applications in the food industry, cinnamic acid has also been studied for its potential therapeutic properties. It is antioxidant and anti-inflammatory, and may also have anticancer properties.

Creative Proteomics is a leading provider of HPLC-MS services for cinnamic acid analysis, using state-of-the-art equipment and technology to provide accurate and reliable results for cinnamic acid analysis. Our platform includes positive and negative ionization modes, multiple reaction monitoring (MRM), and full scan modes to detect and quantify cinnamic acid in a variety of samples, including food, pharmaceuticals, and cosmetics.

The chemical structure of natural and synthetic plant cinnamic acid and resistant plant cinnamic acid Figure 1. The chemical structure of natural and synthetic plant cinnamic acid and resistant plant cinnamic acid (Park 2020).

Detection Methods of Cinnamic Acid Analysis

To detect and quantify cinnamic acid in different samples, high performance liquid chromatography-mass spectrometry (HPLC-MS) is commonly used.

HPLC-MS is a widely used analytical method that involves separating the different components of a sample based on their chemical properties, followed by the detection and identification of cinnamic acid using mass spectrometry.

In HPLC-MS, a sample is first injected into an HPLC system, which separates the different components based on their interaction with the stationary phase. The separated components are then sent to a mass spectrometer, where they are ionized and separated according to their mass-to-charge ratios. The resulting mass spectrum can be used to identify and quantify cinnamic acid in the sample.

HPLC-MS is highly sensitive and selective for the detection of cinnamic acid at low concentrations and in complex matrices, such as food and biological samples, with accurate and reproducible results.

Cinnamic acid analysis service workflowFigure 2. Cinnamic acid analysis service workflow.

Mode: MRM

Precision: pmol

Linear: R2 > 0.99

Analysis content:

  • Standard curve creation
  • Raw data preprocessing
  • Absolute quantitative and qualitative analysis of plant cinnamic acid
  • Differential metabolite screening

Applications of Cinnamic Acid Analysis

Pharmacology: Identify and quantify the active ingredients of cinnamic acid and its derivatives in pharmaceuticals, helping to study the pharmacokinetics and pharmacodynamics of these compounds.

Food Science: Determining the amount of cinnamic acid in different food products, such as cinnamon, is critical to ensure compliance and quality control. In addition, it can also be used to study the bioavailability and metabolism of cinnamic acid in the human body.

Cosmetics Industry: Identify the botanical extracts they use in skin care products, measure the concentration of cinnamic acid in cosmetic formulations to ensure consistency of quality and efficacy.

Natural Product Chemistry: Identify the presence of cinnamic acid in various plant species and exploration of its biosynthetic and metabolic pathways. Screening of natural product extracts for the presence of cinnamic acid and its derivatives.

Advantages of Our Cinnamic Acid Analysis Service:

Flexible statistical analysis and bioinformatics analysis.

Fast turnaround times and affordable prices.

Rich experience in plant sample handling and cinnamic acid analysis.

Accurate detection and quantitation of cinnamic acid with HP-LC platforms.

Fast detection speed, simple detection method, practicality and high accuracy.

List of Cinnamic Acid Compounds We Can Detect

Trans-cinnamic acidCis-cinnamic acidp-Coumaric acidCaffeic acidFerulic acid
Sinapic acidIsoferulic acid4-Hydroxycinnamic acid3,4-Dihydroxycinnamic acid

Sample Requirements of Cinnamic Acid Assay

Sample RequirementDescription
Sample TypesPlant extracts, food products, beverages, cosmetics, pharmaceutical formulations, etc.
Sample VolumeRecommended volume: 5-10 mL for liquid samples, 1-5 g for solid samples
Sample StorageStore samples in amber glass vials or airtight containers. Keep at -20°C for long-term storage.
Sample TransportationShip samples on dry ice to maintain stability. Avoid temperature fluctuations during transit.
Sample PreparationExtraction method: Depending on sample type, use techniques such as maceration, solvent extraction, liquid-liquid extraction, etc.
Sample HomogeneityEnsure homogeneity by thoroughly mixing or grinding the sample.
Sample InformationProvide accurate information about sample origin, composition, processing methods, and any relevant details.


  • Experimental procedures
  • The parameters of liquid chromatograph and mass spectrometer
  • Extract mitochondria and purified protein samples
  • Purity analysis report
  • MS raw data files and MS data quality inspection
  • Metabolite quantitative data
  • Customized analysis report


  1. Lee, YS, Son, E, et al. Synergistic uric acid-lowering effects of the combination of chrysanthemum indicum linne flower and cinnamomum cassia (l.) j. persl bark extracts. Evid Based Compl Alt. 2017.
  2. Park, Y. H. , Kim, D. H. , et al. A 12-week, multicenter, randomized, double-blind, placebo-controlled clinical trial for evaluation of the efficacy and safety of dkb114 on reduction of uric acid in serum. Nutrients, (2020), 12(12), 3794.
For Research Use Only. Not for use in diagnostic procedures.


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