Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Soybean Agglutinin (SBA) Analysis Service

Soybean Agglutinin (SBA) Analysis Service

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About soybean agglutinin (SBA)

Soybean agglutinin (SBA) is mainly present in the protein body of soybean cotyledon cells, about 10% of the total soybean protein.

Soybeans contain two types of soybean lectins, one is one of the main anti-nutritional factors in soybeans, and the second type can specifically recognize and bind rhizobia in soybean roots.

SBA has a wide range of applications, such as probes for studying the distribution of sugar chains on the cell surface, studying the separation and purification of cell membranes and other glycolipids and glycoproteins, studying the separation of cell subgroups with different biological activities, studying the characteristics of tumor cell membranes, etc.

Creative Proteomics' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results.

The technical route of targeted metabolomics of soybean agglutinin (SBA)

Schematic diagram of SBA structure Schematic diagram of SBA structure

The technical route of targeted metabolomics of soybean agglutinin (SBA)

Sample requirements

Sample demand
Plant root / stem / leaf / fruit / seed > 200 mg fresh weight, freeze-dried sample > 0.5 g
Animal tissue / feces related samples > 200mg / sample
Cells / bacteria > 107
Serum / plasma / urine / ruminal fluid / cerebrospinal fluid / amniotic fluid and other homogeneous liquid samples > 200 µl.
(Hemolysis should be avoided. Cerebrospinal fluid can be as low as 100 µl.)
For other sample types, please consult technical support or sales.
Sample mixed and repeat
In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample. (※ The same condition refers to the same period, basically the same phenotype, and the same part.)
It is recommended that prepare more than 6 biological replicates.
Storage and transportation
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature, and store it at -80℃.
Dry ice transportation (about 3~4kg dry ice is consumed every day, please use sufficient dry ice for transportation).

Creative Proteomics offers several approaches to plant metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.

References

  1. Pan L, Mohammed F, Qin G, et al. The Influences of Soybean Agglutinin and Functional Oligosaccharides on the Intestinal Tract of Monogastric Animals[J]. International Journal of Molecular Sciences, 2018, 19(2):554.
  2. Vandenborre G, Smagghe G, Damme E. Plant lectins as defense proteins against phytophagous insects[J]. Phytochemistry, 2011, 72(13):1538-1550.
  3. Yida, Zhang, Xiqing, et al. Metabolomics of clinical poisoning by aconitum alkaloids using derivatization LC-MS.[J]. Frontiers in pharmacology, 2019.
For Research Use Only. Not for use in diagnostic procedures.

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