3-acetylindole Analysis Service

What is 3-Acetylindole?

3-Acetylindole, also known as 1H-indol-3-yl acetate, is a derivative of indole with a acetyl group attached to the nitrogen atom. It is a key intermediate in the synthesis of various organic compounds and serves as a building block in the preparation of pharmaceutical drugs and natural products. The accurate analysis of 3-acetylindole is crucial for quality control, drug discovery, and environmental monitoring. Liquid chromatography-mass spectrometry (LC-MS) has emerged as a powerful technique for the detection, identification, and quantification of 3-acetylindole due to its high sensitivity, selectivity, and versatility.

Molecular structure of 3-acetylindole

3-Acetylindole Analysis Platform in Creative Proteomics

Creative Proteomics provides 3-acetylindole analysis services based on an LC-MS platform. High-performance liquid chromatography (HPLC) provides excellent resolution and separation efficiency, while mass spectrometry offers accurate mass determination and structural elucidation of the analyte.

The HPLC system consists of a pump, injector, column, and detector. Reverse-phase HPLC columns with C18 or C8 stationary phases are commonly employed for the separation of 3-acetylindole. The mobile phase composition can vary depending on the desired separation and detection sensitivity.

The mass spectrometer in the LC-MS system ionizes the analyte molecules using different ionization techniques, such as electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI). ESI is often preferred for the analysis of 3-acetylindole due to its efficiency in generating protonated molecular ions ([M+H]+) and its soft ionization nature.

LC-MS Methods for 3-Acetylindole Analysis

Several LC-MS techniques for analyzing 3-acetylindole in various sample matrices have been developed. The quantitative measurement of 3-acetylindole in medicinal formulations is a common approach. The analyte is extracted from the matrix, followed by HPLC separation and MS detection. Internal standards or external calibration curves are typically used for quantification.

The other is metabolite detection and characterization in biological materials. To get comprehensive structural information about the metabolite, LC-MS is paired with tandem mass spectrometry (LC-MS/MS). Sample preparation, LC separation, MS/MS fragmentation, and data interpretation utilizing specialized software are all part of the analysis.

We can also monitor the presence and fate of 3-acetylindole and its derivatives in environmental materials such as water and soil. Samples are commonly prepared using solid phase extraction (SPE) or liquid-liquid extraction (LLE), followed by LC separation and MS detection.

Applications of LC-MS Analysis for 3-Acetylindole

Pharmaceutical Research and Quality Control

LC-MS analysis allows for accurate quantification of 3-acetylindole, ensuring the consistency and efficacy of pharmaceutical products. By employing LC-MS, researchers can monitor batch-to-batch variations, assess the stability of formulations, and ensure compliance with regulatory standards.

Metabolite Identification and Pharmacokinetic Studies

In the field of metabolomics and drug metabolism research, LC-MS analysis is instrumental in identifying and characterizing the metabolites of 3-acetylindole in biological samples. By coupling LC with tandem mass spectrometry (LC-MS/MS), you can obtain detailed structural information about metabolites, facilitating the understanding of metabolic pathways, pharmacokinetics, and the overall fate of 3-acetylindole in the body.

Environmental Monitoring and Toxicology

Accurately identify and quantify 3-acetylindoles and their derivatives in complex environmental matrices, including analysis of water, soil and air samples to determine levels of these compounds and assess their potential ecological and human health risks.

Natural Product Analysis and Biosynthetic Studies

Natural products containing 3-acetylindole are of great importance in scientific fields such as natural product chemistry and drug development. LC-MS analysis enables the identification, quantification and structural characterization of 3-acetylindole in complex natural product extracts.

Sample Requirements

• Plant samples need to provide more than 1g fresh weight/sample, and freeze-drying is required.
• Animal tissues need to provide more than 200mg of fresh weight/sample, which will be divided into 2-4mm pieces immediately after sampling, and then frozen in liquid nitrogen.
• Plant tissues need to be mixed from more than 3 plant tissues into one sample. If animal tissues need to be mixed, the two parties should communicate in advance.
• Algae provides 30-40mg freeze-dried powder/sample, or more than 300mg fresh sample/sample.
• Blood samples need to be drawn into anticoagulation centrifuge tubes, and then immediately centrifuged at low temperature to collect plasma and blood cells, and finally frozen in liquid nitrogen.
• Samples should be stored at -80°C, transported on dry ice, and cannot be repeatedly frozen and thawed.

Recommended Number of Sample Repetitions

• Animal samples (various tissues, plasma) ≥ 6 replicates/group
• Plant samples and microbial samples (leaf, root tissue) ≥ 3 replicates/group
• Cell sample ≥ 3 replicates/group
• Clinical samples (serum, urine, various tissues, etc.) ≥ 10 cases/group

Feedback to Customers

Creative Proteomics will provide you with detailed technical reports, including

• Experimental steps
• Related mass spectrometry parameters
• Part of the mass spectrum picture
• Raw data
• Quantitative results of targeted metabolites

Project Cycle

• A standard experiment and analysis process takes about 1 to 4 weeks.

Creative Proteomics has leading technology, first-class R&D and production equipment, and has established a complete metabolome service system for plants. Our professional team has many years of experience in metabolomics, bioinformatics, and statistics, and can help you plan, conduct and report metabolomics research, so that your upstream and downstream experiments can be seamlessly connected. We can provide a wide range of target metabolomics services, including target metabolic pathways, target metabolite analysis, etc. You can also perform personalized customization services at the same time during the data analysis stage. If you have any questions or specific requirements, please feel free to contact us.