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Creative Proteomics Metabolomics

Liquiritin Analysis Service

Liquiritin Analysis Service

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Creative Proteomics provides high performance liquid chromatography (HPLC) based methods for liquiritin analysis, which provides sufficient stability, sensitivity and high resolution for identification and quantification of liquiritin. This approach has the advantages of simple, stable, fast, accurate, specific and reproducible, which can help researchers to analyze liquiritin effectively.

Overview

Liquiritin exists in the root of licorice, a leguminous plant. Liquiritin is an important monomer active component in licorice flavonoids, and is an important index for the quality control of licorice medicinal materials and their preparations. Liquiritin is a white powder soluble in methanol, ethanol, hot water and slightly soluble in ethyl acetate. Liquiritin is 100 to 500 times sweeter than sucrose. The sweetness is slow and lasts a long time. When used as sweet taste improver or enhancer, it is usually used in combination with other sweeteners. Clinically, licorice flavonoids have the function of scavenging free radicals, anti-oxidation, anti-cancer promotion and anti-protrusion. Because liquiritin has a wide range of uses, it is very important to establish the method for the analysis of liquiritin in plant metabolites. Creative Proteomics provides the simple, rapid, accurate, affordable and highly automated analytical method and equipment to analyse liquiritin. High performance liquid chromatography (HPLC) can solve some of the problems associated with liquiritin analysis. Improved extraction and purification steps can also increase the sensitivity of the analytical method.

Applications of Liquiritin Analysis

  • Qualitative analysis of the main types of liquiritin
  • Quantitative analysis of liquiritin content
  • Ensure safety and quality
  • Provide basis for improvement

Advantages of Our Liquiritin Analysis Service

Experienced plant metabolomics technical expert. The Creative Proteomics specialists provide the client with a comprehensive liquiritin analysis program to assist the client in quality management.

Comply with relevant regulations. The service of liquiritin analysis in the Creative Proteomics testing laboratory has been carried out for many years. Creative Proteomics strictly complies with the laws and regulations required by customers, helping customers provide professional reports for liquiritin analysis.

High cost performance. With high-quality service and professional and efficient operation management, Creative Proteomics provides customers with cost-effective liquiritin analysis services and makes a positive contribution to ensuring safety and quality.

Service Workflow

Our newly developed sample preprocessing method provides safety, environmental protection, rapidness, effectiveness, and the average recovery rate is over 90%. Sample preprocessing at Creative Proteomics complies with the in-house standard operation procedures (SOP), ensuring maximized homogeneity between samples.

Liquiritin analysis service workflow;Figure 1. Liquiritin analysis service workflow.

Analysis Methods: dual wavelength reversed-phase HPLC

Mobile phase: acetonitrile-phosphoric acid solution

Detector: UV detector

Linear range: r>0.999

Time: dozens of minutes, slightly different according to different ingredients

Analysis content:

  • Standard curve creation
  • Raw data preprocessing
  • Absolute quantitation of liquiritin
  • Differential metabolite screening
  • Optimal analyses

Sample Requirements

1. Fresh tissues >1 g. If possible, samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.

2. Product samples.

At least 3 biological replicates.

Delivery

  • Experimental procedure
  • Parameters of HPLC
  • Purity analysis report
  • HPLC raw data files and HPLC data quality checks
  • Metabolites quantification data
  • Custom analysis report

HPLC based profiling of liquiritin enables quantitative analyses in a faster, more convenient, and more sensitive manner. With decades of experience in HPLC services, Creative Proteomics has a proven track record supporting diverse liquiritin detection and quantitation. We can meet your specific project requirements, from sample to bioinformatics.

For Research Use Only. Not for use in diagnostic procedures.

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