Creative Proteomics provides high performance liquid chromatography (HPLC) based methods for hesperetin analysis, which provides sufficient stability, sensitivity and high resolution for identification and quantification of hesperetin. This approach has the advantages of simple, stable, fast, accurate, specific and reproducible, which can help researchers to analyze hesperetins effectively.
Hesperetin is a chemical derived primarily from young citrus fruits in the rutin family. Hesperetin is a member of the dihydroflavones group and is usually a pale yellow needle crystal or powder. Hesperetin is insoluble in water, soluble in methanol, ethanol, acetone and other organic solvents. Hesperetin has antioxidant, anti-inflammatory, hypolipidemic, anti-tumor and cardiovascular protective effects, and can be used in health care drugs and health products, some cosmetics additives and food. In addition, hesperetin as a drug raw material, the relevant drugs can be used for stomach-strengthening, expectorant, cough suppression, wind drive, diuresis, antiretrograde and stomachache. Because hesperetin has a wide range of uses, it is very important to establish the method for the analysis of hesperetin in plant metabolites. Creative Proteomics provides the simple, rapid, accurate, affordable and highly automated analytical method and equipment to analyse hesperetin. High performance liquid chromatography (HPLC) can solve some of the problems associated with hesperetin analysis. Improved extraction and purification steps can also increase the sensitivity of the analytical method.
Applications of Hesperetin Analysis
- Qualitative analysis of the main types of hesperetin
- Quantitative analysis of hesperetin content
- Ensure safety and quality
- Provide basis for improvement
Advantages of Our Hesperetin Analysis Service
Excellent hesperetin qualitative and quantitative capabilities. We have automatic analysis system, which can efficiently and accurately complete qualitative and quantitative analysis, ensuring that the results are scientific and reliable.
Individualized experimental scheme. We can provide one-to-one customized services, assign professional project leaders to analyze the whole project, directly and quickly report the progress to customers, and flexibly adjust the project plan according to customers' opinions.
Full cycle quality control system
Professional data analysis skills
Our newly developed sample preprocessing method provides safety, environmental protection, rapidness, effectiveness, and he average recovery rate is up to 99%. Sample preprocessing at Creative Proteomics complies with the in-house standard operation procedures (SOP), ensuring maximized homogeneity between samples.
Figure 1. Hesperetin analysis service workflow.
Mobile phase: acetonitrile - water
Detector: UV detector
Linear range: r>0.999
Time: dozens of minutes, slightly different according to different ingredients
- Standard curve creation
- Raw data preprocessing
- Absolute quantitation of hesperetin
- Differential metabolite screening
- Optimal analyses
1. Fresh tissues >1 g. If possible, samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.
2. Product samples.
At least 3 biological replicates.
- Experimental procedure
- Parameters of HPLC
- Purity analysis report
- HPLC raw data files and HPLC data quality checks
- Metabolites quantification data
- Custom analysis report
HPLC based profiling of hesperetin enables quantitative analyses in a faster, more convenient, and more sensitive manner. With decades of experience in HPLC services, Creative Proteomics has a proven track record supporting diverse hesperetin detection and quantitation. We can meet your specific project requirements, from sample to bioinformatics.
For Research Use Only. Not for use in diagnostic procedures.