Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Diphtheria Toxin Analysis Service

Diphtheria Toxin Analysis Service

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Creative Proteomics' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results.

About diphtheria toxin

Diphtheria toxin is a protein that has the characteristics of toxicity and specific immunity. Biological missiles (targeted drugs) are commonly used as factors to kill tumor cells.

Diphtheria toxin can cause biological nerve demyelination.

Diphtheria toxin has the effect of inhibiting protein synthesis. By inhibiting translocation reaction, it mainly inhibits protein synthesis in mammals.

Diphtheria toxin precursorDiphtheria toxin precursor

The technical route of targeted metabolomics of diphtheria toxin

The technical route of targeted metabolomics of diphtheria toxin

Sample requirements

Sample requirements
Sample type
Cell sample - please centrifuge after sampling, remove the medium, immediately put it in liquid nitrogen, and store it at -80℃.
Tissue sample - please put it in liquid nitrogen immediately after separation and store it at -80℃.
Sample mixed
In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
※ The same condition refers to the same period, basically the same phenotype, and the same part.
Sample weight and repeat
Samples that have been lyophilized are >0.5g.
For samples with low water content, such as stems, flowers, seeds, dormant buds, etc., fresh samples >1g.
For samples with middle water content, roots and leaves (especially young leaves), fresh samples >2g.
For samples with water content >90%, if fruit (tomato, watermelon, citrus, etc.), fresh sample >5g.
Blood ≥100 µl, Urine ≥100 μl, Tissue ≥50 mg, Fresh stool ≥50 mg, Freeze-dried stool ≥5 mg, Cell ≥1*107
It is recommended that prepare more than 6 biological replicates.
Storage and transportation
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature.
Dry ice transportation (about 3~4kg dry ice is consumed every day, please use sufficient dry ice for transportation).
There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.

Creative Proteomics offers several approaches to plant metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.

References

  1. Bhatnagar D, Rajasekaran K, Payne G, et al. The'omics' tools: genomics, proteomics, metabolomics and their potential for solving the aflatoxin contamination problem[J]. World Mycotoxin Journal, 2008, 1(1): 3-12.
  2. Falade T, Chrysanthopoulos P K, Hodson M P, et al. Metabolites identified during varied doses of Aspergillus species in Zea mays grains, and their correlation with aflatoxin levels[J]. Toxins, 2018, 10(5): 187.
  3. Mohammed A, Hussain R M, Ur R N, et al. De novo transcriptome sequencing and metabolite profiling analyses reveal the complex metabolic genes involved in the terpenoid biosynthesis in Blue Anise Sage (Salvia guaranitica L.). DNA Research (6):6.
For Research Use Only. Not for use in diagnostic procedures.
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