Pyrimidine Metabolism Service

Overview

Pyrimidine nucleotides play a crucial role in cellular metabolism serving as activated precursors of RNA and DNA, CDP diacylglycerol phosphoglyceride for the maintenance of cell membranes and UDP-sugars for protein glycosylation and glycogen synthesis. In essence, pyrimidine metabolism is a branch of nucleotide metabolism that produces nucleosides and deoxy/ribonucleotides of the pyrimidine bases including cytosine, thymine and uracil. Basically, pyrimidine metabolism comprises three pathways: 1) salvaging of free nucleosides and bases, 2) the de novo synthesis from amino acids and ribose precursors, and 3) the catabolism of excess nucleoside and nucleotides. The any disruption of these three pathways will result in the aberrant pyrimidine metabolism which is closely associated with a wide range of human pathologies like cancer and hereditary orotic aciduria. Scientists at Creative Proteomics take advantage of highly quantitative methods with high-performance liquid chromatography (HPLC) for the determination of pyrimidine metabolism related metabolites levels in various samples. Furthermore, we provide reliable, rapid and cost-effective pyrimidine metabolism related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The Main Applications of Pyrimidine Metabolism Service

• Identifying novel biomarkers of diseases
• Improving diagnose diseases
• Accelerating development of novel drugs
• Gaining new mechanism of diseases

Advantages of Our Pyrimidine Metabolism Service

• Constantly optimized protocol and analytical software
• Professional experiment design
• Quick turnaround time
• High accuracy, specificity, and sensitivity

Service Workflow

Creative Proteomics provides alpha-linolenic acid metabolism analysis service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography- mass spectrometry (HPLC-MS) platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC-MS data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

Figure 1. The overall workflow of pyrimidine metabolism

List of Partial Detectable Pyrimidine Metabolism Related Metabolites at Creative Proteomics

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from animals. If you need transport your samples to us, please follow the following requirements for different types of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
  Shipment condition: dry ice

Report Delivery

• Experimental protocols
• Instrumental factors of HPLC
• The raw data files of HPLC and the summary of HPLC data quality
• Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of pyrimidine metabolism, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored pyrimidine metabolism analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

1. Löffler Monika, Fairbanks Lynette D, Zameitat Elke, et al. Pyrimidine pathways in health and disease. Trends Mol Med. 2005.11:430-437.
2. Siddiqui Aarif, Ceppi Paolo. A non-proliferative role of pyrimidine metabolism in cancer. Mol Metab. 2020.35:100962.